The sympathetic nervous system may have originated in jawless fish—not tens of millions of years later as previously thought, according to a study published today in Nature.
Anatomical work dating back to the 19th century suggested that the sympathetic nervous system was present only in jawed vertebrates. Yet the sea lamprey, the new findings reveal, sports clusters of sympathetic neurons along its trunk and expresses several genes involved in the “fight-or-flight” system, the response that kicks into gear when an animal perceives a threat.
“Whenever new research causes troves of textbooks to need corrections, that’s always surprising,” says Tyler Square, assistant professor of molecular genetics at the University of Florida, who was not involved in the study.
The team behind the new work decided to re-examine conventional wisdom after a postdoctoral researcher in the lab produced microscopy images of lamprey embryos stained for neurons in the animals’ gut. The stain highlighted some “small sort of concentrations of cells” that looked a lot like sympathetic neurons, recalls lead investigator Marianne Bronner, professor of biology at the California Institute of Technology.
“I said, ‘Oh, those shouldn’t be there.’ So then we decided to delve deeper into it.”
T
he unexpected neurons express several key genes—specifically ASCL1, PHOX2 and HAND—involved in the sympathoadrenal system, the team discovered using a suite of techniques, including immunohistochemistry, in situ hybridization chain reaction and RNA sequencing.These are “all transcription factors that are known to be important in sympathetic neuron differentiation in mammals,” Bronner says.
The cells also produce an enzyme called tyrosine hydroxylase (TH) that is crucial for the synthesis of noradrenaline, which is released during the fight-or-flight response. A subset of these TH-positive cells derive from the animals’ neural crest, subsequent cell-fate-mapping experiments revealed.