Complete picture: Researchers across 12 different labs recorded from almost 300 brain areas during a decision-making task, creating the most comprehensive map of mouse brain activity to date.
International Brain Laboratory

Everything everywhere all at once: Decision-making signals engage entire brain

The findings, gleaned from the most comprehensive map yet of brain activity during decision-making in mice, show that the process is even more distributed than previously thought.

By Claudia López Lloreda
3 September 2025 | 5 min read

The process of making a decision engages neurons across the entire brain, according to a new mouse dataset created by an international collaboration.

“Many, many areas are recruited even for what are arguably rather simple decisions,” says Anne Churchland, professor of neurobiology at University of California, Los Angeles and one of the founding members of the collaboration, called the International Brain Laboratory (IBL).

The canonical model suggests that the activity underlying vision-dependent decisions goes from the visual thalamus to the primary visual cortex and association areas, and then possibly to the frontal cortex, Churchland says. But the new findings suggest that “maybe there’s more parallel processing and less of a straightforward circuit than we thought.”

Churchland and other scientists established the IBL in 2017 out of frustration with small-scale studies of decision-making that analyzed only one or two brain regions at a time. The IBL aimed to study how the brain integrates information and makes a decision at scale. “We came together as a large group with the realization that a large team effort could be transformative in these questions that had been kind of stymieing all of us,” Churchland says.

After years of standardizing their methods and instrumentation across the 12 participating labs, the IBL team constructed a brain-wide map of neural activity in mice as they complete a decision-making task. That map, published today in Nature, reveals that the activity associated with choices and motor actions shows up widely across the brain. The same is true for the activity underlying decisions based on prior knowledge, according to a companion paper by the same team, also published today in Nature.

The IBL labs generated the map by pooling their Neuropixels recordings from 139 mice that completed a visual discrimination task. Each lab recorded different brain areas, but together they covered the entire brain. The resulting dataset spans almost 300 brain areas and includes activity from more than 620,000 neurons, making it one the richest datasets on decision-making, says Floris de Lange, professor of brain, cognition and behavior at Radboud University, who was not involved with the study.

The IBL has made the dataset public, which de Lange says enables researchers to “test new ideas in a really easy and powerful way.”

T

he IBL selected a visual behavioral task complex enough to get at the different aspects of decision-making but simple enough to reproduce across labs.

During the task, mice saw a black-and-white-striped circle appear on the left or the right side of a screen and then maneuvered a tiny wheel to center the circle on the screen. The mice received a sip of water if they succeeded and heard white noise if they failed. Electrodes recorded brain activity as mice observed the visual cue, made their choice, moved the wheel and received the water or noise feedback.

As soon as the animals saw the visual cue, activity appeared in predictable areas, including the visual cortex, the thalamus and the prefrontal cortex.

Distributed decisions: Choices elicit activity in a broad array of brain areas, some of which are still unexplored in the field.
International Brain Laboratory

Activity associated with choice showed up in cortical areas, in line with previous findings, but it also occurred in subcortical areas such as the hindbrain and cerebellum, challenging the notion that only a few select areas encode information about decision-making and supporting the idea that it is widespread.

“It is interesting how much choice selectivity is everywhere,” says Long Ding, research associate professor of neuroscience at the University of Pennsylvania, who was not involved in the work.

Movement- and feedback-related signals also pervaded across the brain: 81 percent of recorded brain regions contained information that could predict the animal’s wheel speed, and activity from nearly all recorded brain regions—including those beyond the associated reward areas—accurately predicted whether the mouse had received a reward or not, with stronger activity in the thalamus, the midbrain and the hindbrain.

If the mice saw the circle more often on one side of the screen than the other, they eventually integrated that prior information into their next decision. This information was represented broadly across 20 to 30 percent of the brain, including in sensory processing areas, such as the dorsal lateral geniculate, that are located early in the visual pathway, the team reported in the second study.

The findings contradict the long-standing idea that prior information is integrated into the process only in higher-order cortical or decision-making regions “at the very last step,” Churchland says. Instead, priors shape decisions all along, the new findings suggest.

A

ltogether, the studies suggest that the current model of decision-making and the brain regions that control it might be limited in scope and that other, unexplored brain areas might also be important, Churchland says.

And although the analyses show that a distributed network of brain regions contains information about decision-making even at early stages of sensory processing, the results do not show causality, so future studies need to determine how that information is used, Ding says. “Yes, [the information is] reflected everywhere, but where is it actually used for the next decision, for learning?”

The comprehensive map sets the stage for those next experiments and could even act as a “library” to help neuroscientists double-check results in their own labs, de Lange says, and ultimately, these studies underscore the importance of large-scale, multilab efforts, particularly for studying brain activity.

The global consortium has since expanded to include 21 experimental and theoretical neuroscience labs and has established a new group called IBL 2.0 that plans to share the tools and expertise it has amassed with new partners, Churchland says. “I hope that our work makes clear that when larger groups of folks team up, they can accomplish things that are beyond the scale of a single laboratory and that really generate critical insights for the field.”

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