A drosophila connectome.
Lighting the way: By revealing unexpected connections between brain regions, the connectome has accelerated hypothesis generation and spurred new collaborations among Drosophila researchers.
Courtesy of Anita Devineni

One year of FlyWire: How the resource is redefining Drosophila research

We asked nine neuroscientists how they are using FlyWire data in their labs, how the connectome has transformed the field and what new tools they would like to see in the future.

Last week marked the first anniversary of the publication of FlyWire, the first-ever complete connectome of an adult female fruit fly brain. The landmark achievement, published in a collection of papers in Nature, was the culmination of a years-long effort spearheaded by the labs of Mala Murthy and H. Sebastian Sung at Princeton University, and the FlyWire consortium, a community of neurobiologists, computer scientists and statisticians at more than 100 institutions around the world.

Mapping more than 100,000 neurons and 50 million synapses, FlyWire provides researchers with a full view of the adult female Drosophila melanogaster nervous system, dramatically speeding the pace of research. Before FlyWire, “people might spend their entire Ph.D. trying to trace the anatomy of a circuit before they could even begin to study its function,” says Anita Devineni, assistant professor of biology at Emory University. “Now we can do this with a few clicks—so we immediately know what the circuit looks like, and we can move on to the more interesting problem of studying how it works.”

Researchers have already used FlyWire data in an array of studies, describing new circuits involved in motor control and revealing novel mechanisms that regulate fly social behaviors and taste sensory processing, for example. “It’s been transformative; FlyWire has dramatically accelerated hypothesis generation and circuit mapping by making whole-brain connectivity accessible and searchable,” says Carolina Rezaval, associate professor of neurogenetics at the University of Birmingham. “The level of anatomical resolution and completeness also lets us think more holistically about how distributed networks coordinate behavior.”

With the connectome in hand, researchers are thinking about new tools and maps that can build on it. “I think the next big step in the connectome domain is to map the chemical connectome,” says Sung Soo Kim, assistant professor in the molecular, cellular and developmental biology department at the University of California, Santa Barbara. Other items on researchers’ wish lists include mapping connectomes from more fly species, overlaying RNA sequencing data on the connectome and integrating FlyWire with common tools, such as NeuronBridge.

To celebrate the first anniversary of FlyWire, we asked nine Drosophila researchers to share how they are using connectome data in their work, what the connectome did for the field and what new tools and resources they want for the future of Drosophila connectomics.

Responses have been lightly edited for length and clarity.

How are you using FlyWire in your research?

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